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Views Read Edit View history. The expression vector is transformed or transfected into the host cell for protein synthesis. Originally Posted by Drewsky Could you tell me how to be a douchebag next? Why would they steal the mail? Smaller plasmids can be used to carry longer segments of foreign DNA. This page was last edited on 27 March , at
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Cloning vector


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Molecular Biology Vector Sequence Database


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Vector (molecular biology)

Insertion vectors contain a unique cleavage site whereby foreign DNA with size of 5—11 kb may be inserted. Host-cell enzymes bind to ORI, initiating replication of the circular plasmid. Uptake of plasmids by E. Auxotrophic selection markers that allow an auxotrophic organism to grow in minimal growth medium may also be used; examples of these are LEU2 and URA3 which are used with their corresponding auxotrophic strains of yeast.
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Bukkake plasmid vector
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